Padlock Probes: Circularizing Oligonucleotides for Localized DNA Detection

作者：Mats F. Nilsson, Helena Malmgren, Martina K. Samiotaki, Marek Kwiatkowski, Bhanu P. Chowdhary, Ulf D. Landegren · 发表于：Science · 年份：1994 · DOI：10.1126/science.7522346 · 被引用次数：801 · 研究领域：Advanced biosensing and bioanalysis techniques、DNA and Nucleic Acid Chemistry、Cystic Fibrosis Research Advances

Nucleotide sequence information derived from DNA segments of the human and other genomes is accumulating rapidly. However, it frequently proves difficult to use such short DNA segments to identify clones in genomic libraries or fragments in blots of the whole genome or for in situ analysis of chromosomes. Oligonucleotide probes, consisting of two target-complementary segments, connected by a linker sequence, were designed. Upon recognition of the specific nucleic acid molecule the ends of the probes were joined through the action of a ligase, creating circular DNA molecules catenated to the target sequence. These probes thus provide highly specific detection with minimal background.