Two-color immunofluorescence using a fluorescence-activated cell sorter.

作者：M R Loken, David R. Parks, L A Herzenberg · 发表于：Journal of Histochemistry & Cytochemistry · 年份：1977 · DOI：10.1177/25.7.330738 · 被引用次数：194 · 研究领域：Diabetes and associated disorders、Monoclonal and Polyclonal Antibodies Research、Lipid Membrane Structure and Behavior

A technique for the analysis of fluorescein and rhodamine in a flow system using a single wavelength of excitation is described. Both optics and electronics are used to discriminate the fluorescein and rhodamine signals. This technique has been used to study the relationship between immunoglobulin M and immunoglobulin D on mouse splenic lymphocytes.