A Functional Genomics Approach to Tanshinone Biosynthesis Provides Stereochemical Insights

作者：Wei Gao, Matthew L. Hillwig, Luqi Huang, Guanghong Cui, Xueyong Wang, Jian‐Qiang Kong, Bin Yang, Reuben J. Peters · 发表于：Organic Letters · 年份：2009 · DOI：10.1021/ol902051v · 被引用次数：268 · 研究领域：Plant biochemistry and biosynthesis、Traditional Chinese Medicine Analysis、Natural product bioactivities and synthesis

Tanshinones are abietane-type norditerpenoid quinone natural products that are the bioactive components of the Chinese medicinal herb Salvia miltiorrhiza Bunge. The initial results from a functional genomics-based investigation of tanshinone biosynthesis, specifically the functional identification of the relevant diterpene synthases from S. miltiorrhiza, are reported. The cyclohexa-1,4-diene arrangement of the distal ring poises the resulting miltiradiene for the ensuing aromatization and hydroxylation to ferruginol suggested for tanshinone biosynthesis.