[11C]Methylation on a C18 Sep-Pak cartridge: a convenient way to produce [N-methyl-11C]choline

作者：Claudio Pascali, Anna Bogni, Ren Itawa, Mara Cambi�, Emilio Bombardieri · 发表于：Journal of Labelled Compounds and Radiopharmaceuticals · 年份：2000 · DOI：10.1002/(sici)1099-1344(200002)43:2<195::aid-jlcr316>3.0.co;2-p · 被引用次数：105 · 研究领域：Medical Imaging Techniques and Applications、Chemical Reactions and Isotopes、Fluorine in Organic Chemistry

[N-Methyl-11C]choline has been simply and efficiently labelled at room temperature by solid supported [11C]methylation of 2-dimethylaminoethanol on a commercial C18 Sep-Pak Light cartridge. The labelled product was retained on a cation exchange resin (Sep-Pak Plus Accell Plus CM) and washed with ethanol and water to remove any excess precursor. Then [N-methyl-11C]choline was desorbed from the cartridge by elution with saline, passed through a 0·2 μm sterile filter and collected in a sterile vial. Overall, the total synthesis time was 12 min from the end of bombardment (EOB). The radiochemical yield from [11C]CO2, decay corrected (EOB) was 87% and the radiochemical purity was greater than 99%. Very conveniently, the automated apparatus employed is a very simple modification of the system routinely used for the synthesis of L-[S-methyl-11C]methionine. Copyright © 2000 John Wiley & Sons, Ltd.