Comparison of oral microbiota in tumor and non-tumor tissues of patients with oral squamous cell carcinoma

作者：Smruti Pushalkar, Xiaojie Ji, Yihong Li, Cherry L. Estilo, Yegnanarayana Ramanathan, Bhuvanesh Singh, Xin Li, Deepak B. Saxena · 发表于：BMC Microbiology · 年份：2012 · DOI：10.1186/1471-2180-12-144 · 被引用次数：347 · 研究领域：Oral microbiology and periodontitis research、Gut microbiota and health、Salivary Gland Disorders and Functions

BACKGROUND: Bacterial infections have been linked to malignancies due to their ability to induce chronic inflammation. We investigated the association of oral bacteria in oral squamous cell carcinoma (OSCC/tumor) tissues and compared with adjacent non-tumor mucosa sampled 5 cm distant from the same patient (n = 10). By using culture-independent 16S rRNA approaches, denaturing gradient gel electrophoresis (DGGE) and cloning and sequencing, we assessed the total bacterial diversity in these clinical samples. RESULTS: DGGE fingerprints showed variations in the band intensity profiles within non-tumor and tumor tissues of the same patient and among the two groups. The clonal analysis indicated that from a total of 1200 sequences characterized, 80 bacterial species/phylotypes were detected representing six phyla, Firmicutes, Bacteroidetes, Proteobacteria, Fusobacteria, Actinobacteria and uncultivated TM7 in non-tumor and tumor libraries. In combined library, 12 classes, 16 order, 26 families and 40 genera were observed. Bacterial species, Streptococcus sp. oral taxon 058, Peptostreptococcus stomatis, Streptococcus salivarius, Streptococcus gordonii, Gemella haemolysans, Gemella morbillorum, Johnsonella ignava and Streptococcus parasanguinis I were highly associated with tumor site where as Granulicatella adiacens was prevalent at non-tumor site. Streptococcus intermedius was present in 70% of both non-tumor and tumor sites. CONCLUSIONS: The underlying changes in the bacterial dive...