Dissemination amongst humans and food products of animal origin of a Salmonella typhimurium clone expressing an integron-borne OXA-30 -lactamase

作者：Patrı́cia Antunes · 发表于：Journal of Antimicrobial Chemotherapy · 年份：2004 · DOI：10.1093/jac/dkh333 · 被引用次数：53 · 研究领域：Salmonella and Campylobacter epidemiology、Antibiotic Resistance in Bacteria、Antimicrobial agents and applications

OBJECTIVES: Characterization of the molecular basis for beta-lactam resistance and evaluation of the clonal relatedness among nine isolates of multidrug-resistant Salmonella typhimurium recovered from seven clinical human samples and two pork end products. METHODS: The isolates were examined for susceptibility to antimicrobial agents. The relationships between resistance genes, class 1 integrons, plasmids and isolates were screened by molecular methods such as polymerase chain reaction and restriction fragment length analysis. RESULTS: A bla(OXA-30) gene, located in a class 1 integron, was detected in all isolates. This integron was present on a conjugative plasmid in all but one isolate. By pulsed-field gel electrophoresis, it was determined that all strains share the same chromosomal type. CONCLUSIONS: This study demonstrates the spread of an OXA-30-producing S. typhimurium in Portugal, suggesting dissemination of a resistant clone through the food chain.