Studies of the hemopoietic microenvironment. III. Differences in the splenic microvascular system and stroma between Sl/Sld and W/Wv anemic mice

作者：Robert S. McCuskey, Howard A. Meineke · 发表于：American Journal of Anatomy · 年份：1973 · DOI：10.1002/aja.1001370205 · 被引用次数：32 · 研究领域：Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities、Renal Diseases and Glomerulopathies

Abstract The differences in the splenic microvascular system and stroma between Sl/Sld and W/Wv anemic mice were investigated using in vivo microscopic and histochemical methods. Spleens of Sl/Sld anemic mice had increased numbers of sinusoids containing blood flow and an increased concentration of sulfated acid mucopolysaccharide in the splenic stroma when compared to spleens of non‐anemic littermates; in both groups of mice the undifferentiated hemopoietic cells in the red pulp were enveloped with a coating of sulfated acid mucopolysaccharide. In contrast, in the W/Wv anemic mice, the number of sinusoids containing blood flow was one‐half of that seen in non‐anemic littermates. While the concentration of mucopolysaccharides in the splenic stroma of the W/Wv mice and their non‐anemic littermates appeared similar, the W/Wv mice had a deficiency in the number of undifferentiated hemopoietic cells in the red pulp as well as a deficiency in the number of such cells enveloped with a coating of sulfated acid mucopolysaccharide. Transfusion of the W/Wv mice with stem cells from non‐anemic littermates corrected the anemia. Blood flow through the sinusoids was restored to near normal levels, and the number of undifferentiated hemopoietic cells increased in the red pulp; most were enveloped with a coating of sulfated acid mucopolysaccharide. The histochemical results suggest specific defects in the composition of mucopolysaccharides in the hemopoietic microenvironment of Sl/Sld anemic...