Wood Cellulose Preparation Methods and Mass Spectrometric Analyses of δ13C, δ18O, and Nonexchangeable δ2H Values in Cellulose, Sugar, and Starch: An Interlaboratory Comparison

作者：Tatjana Boettger, Marika Haupt, Kay Knöller, Stephan M. Weise, John S. Waterhouse, Katja T. Rinne‐Garmston, Neil J. Loader, Eloni Sonninen, H. Jungner, Valérie Masson‐Delmotte, M. Stiévenard, M. T. Guillemin, Monique Pierre, Anna Pazdur, Markus Leuenberger, Marc S. Filot, Matthias M Saurer, Christina E. Reynolds, Gerd Helle, Gerhard Hans Schleser · 发表于：Analytical Chemistry · 年份：2007 · DOI：10.1021/ac0700023 · 被引用次数：225 · 研究领域：Tree-ring climate responses、Isotope Analysis in Ecology、Archaeology and ancient environmental studies

Interlaboratory comparisons involving nine European stable isotope laboratories have shown that the routine methods of cellulose preparation resulted in data that generally agreed within the precision of the isotope ratio mass spectrometry (IRMS) method used: +/-0.2 per thousand for carbon and +/-0.3 per thousand for oxygen. For carbon, the results suggest that holocellulose is enriched up to 0.39 per thousand in 13C relative to the purified alpha-cellulose. The comparisons of IRMS measurements of carbon on cellulose, sugars, and starches showed low deviations from -0.23 to +0.23 per thousand between laboratories. For oxygen, IRMS measurements varied between means from -0.39 to 0.58 per thousand, -0.89 to 0.42 per thousand, and -1.30 to 1.16 per thousand for celluloses, sugars, and starches, respectively. This can be explained by different effects arising from the use of low- or high-temperature pyrolysis and by the variation between laboratories in the procedures used for drying and storage of samples. The results of analyses of nonexchangeable hydrogen are very similar in means with standard deviations between individual methods from +/-2.7 to +/-4.9 per thousand. The use of a one-point calibration (IAEA-CH7) gave significant positive offsets in delta2H values up to 6 per thousand. Detailed analysis of the results allows us to make the following recommendations in order to increase quality and compatibility of the common data bank: (1) removal of a pretreatment with organic...