Arginase I Expression and Activity in Human Mononuclear Cells After Injury

作者：Juan B. Ochoa, Andrew C. Bernard, William Eugene O’Brien, Margaret Mary Griffen, Mary E. Maley, Anna K. Rockich, Betty J. Tsuei, Bernard R. Boulanger, Paul A. Kearney, Sidney M. Morris · 发表于：Annals of Surgery · 年份：2001 · DOI：10.1097/00000658-200103000-00014 · 被引用次数：165 · 研究领域：Nitric Oxide and Endothelin Effects、Cancer Research and Treatments、Sulfur Compounds in Biology

OBJECTIVE: To determine the effect of trauma on arginase, an arginine-metabolizing enzyme, in cells of the immune system in humans. SUMMARY BACKGROUND DATA: Arginase, classically considered an enzyme exclusive to the liver, is now known to exist in cells of the immune system. Arginase expression is induced in these cells by cytokines interleukin (IL) 4, IL-10, and transforming growth factor beta, corresponding to a T-helper 2 cytokine profile. In contrast, nitric oxide synthase expression is induced by IL-1, tumor necrosis factor, and gamma interferon, a T-helper 1 cytokine profile. Trauma is associated with a decrease in the production of nitric oxide metabolites and a state of immunosuppression characterized by an increase in the production of IL-4, IL-10, and transforming growth factor beta. This study tests the hypothesis that trauma increases arginase activity and expression in cells of the immune system. METHODS: Seventeen severely traumatized patients were prospectively followed up in the intensive care unit for 7 days. Twenty volunteers served as controls. Peripheral mononuclear cells were isolated and assayed for arginase activity and expression, and plasma was collected for evaluation of levels of arginine, citrulline, ornithine, nitrogen oxides, and IL-10. RESULTS: Markedly increased mononuclear cell arginase activity was observed early after trauma and persisted throughout the intensive care unit stay. Increased arginase activity corresponded with increased argina...