Genomic Analysis of Gene Expression in C. elegans

作者：Andrew A. V. Hill, Craig P. Hunter, B. T. Tsung, Greg Tucker‐Kellogg, Eugene L. Brown · 发表于：Science · 年份：2000 · DOI：10.1126/science.290.5492.809 · 被引用次数：300 · 研究领域：Genetics, Aging, and Longevity in Model Organisms、Bioinformatics and Genomic Networks、CRISPR and Genetic Engineering

Until now, genome-wide transcriptional profiling has been limited to single-cell organisms. The nematode Caenorhabditis elegans is a well-characterized metazoan in which the expression of all genes can be monitored by oligonucleotide arrays. We used such arrays to quantitate the expression of C. elegans genes throughout the development of this organism. The results provide an estimate of the number of expressed genes in the nematode, reveal relations between gene function and gene expression that can guide analysis of uncharacterized worm genes, and demonstrate a shift in expression from evolutionarily conserved genes to worm-specific genes over the course of development.