Disappearance of the Lymphoid System in Bcl-2 Homozygous Mutant Chimeric Mice

作者：Kei-ichi Nakayama, Keiko Nakayama, Izumi Negishi, Keisuke Kuida, Yoichi Shinkai, Marjorie C. Louie, Larry E. Fields, Phillip J. Lucas, Valerie Stewart, Frederick W. Alt, Dennis Y. Loh · 发表于：Science · 年份：1993 · DOI：10.1126/science.8372353 · 被引用次数：404 · 研究领域：T-cell and B-cell Immunology、Immune Cell Function and Interaction、Immunotherapy and Immune Responses

The bcl-2 proto-oncogene can prevent the death of many cell types. Mice were generated that were chimeric for the homozygous inactivation of bcl-2. Lymphocytes without Bcl-2 differentiated into phenotypically mature cells. However, in vitro, the mature T cells that lacked Bcl-2 had shorter life-spans and increased sensitivity to glucocorticoids and gamma-irradiation. In contrast, stimulation of CD3 inhibited the death of these cells. T and B cells with no Bcl-2 disappeared from the bone marrow, thymus, and periphery by 4 weeks of age. Thus, Bcl-2 was dispensable for lymphocyte maturation, but was required for a stable immune system after birth.