Absolute Linkage of Virulence and Central Nervous System Cell Tropism of Reoviruses to Viral Hemagglutinin

作者：Howard L. Weiner, M. Linda Powers, Bernard N. Fields · 发表于：The Journal of Infectious Diseases · 年份：1980 · DOI：10.1093/infdis/141.5.609 · 被引用次数：220 · 研究领域：Viral gastroenteritis research and epidemiology、Virus-based gene therapy research、Respiratory viral infections research

That the hemagglutinin (HA) of reovirus, encoded in the S1 gene, determines the central nervous system (CNS) cell tropism of reovirus type 1 and 3 was shown using recombinant clones containing nine genes from one serotype and the S1 gene from the other. Clone 1.HA3 contains nine genes from type 1 and the S1 gene from type 3; 3.HA1 is the reciprocal clone. Type 3 and 1.HA3 cause a fatal encephalitis in newborn mice with neuronal destruction but no ependymal cell damage, whereas type 1 and 3.HA1 cause a nonfatal ependymal infection but no neuronal damage. Immunofluorescent studies showed no viral antigen in ependymal cells of mice infected with type 3 or 1.HA3 or in neuronal cells of mice infected with type 1 or 3.HA1. With type 3 or clones containing the type 3 HA, maximal brain titers were 10(10) plaque-forming units; maximal titers were 10(8) plaque-forming units for type 1 or clones containing the type 1 HA. This pattern of reovirus virulence for CNS probably relates to the specific interaction of viral HA with neuronal or ependymal surface receptors.