A Calmodulin-Related Protein That Suppresses Posttranscriptional Gene Silencing in Plants

作者：Radhamani Anandalakshmi, RAJENDRA P. MARATHE, Xin Ge, J. M. Herr, Christopher J. D. Mau, Allison Mallory, Gail J. Pruss, Lewis H. BOWMAN, Vicki Bowman Vance · 发表于：Science · 年份：2000 · DOI：10.1126/science.290.5489.142 · 被引用次数：350 · 研究领域：Plant Virus Research Studies、Plant and Fungal Interactions Research、Plant Disease Resistance and Genetics

Posttranscriptional gene silencing (PTGS) is an ancient eukaryotic regulatory mechanism in which a particular RNA sequence is targeted and destroyed. The helper component-proteinase (HC-Pro) of plant potyviruses suppresses PTGS in plants. Using a yeast two-hybrid system, we identified a calmodulin-related protein (termed rgs-CaM) that interacts with HC-Pro. Here we report that rgs-CaM, like HC-Pro itself, suppresses gene silencing. Our work is the first report identifying a cellular suppressor of PTGS.