Generation of a Large Combinatorial Library of the Immunoglobulin Repertoire in Phage Lambda

作者：William D. Huse, Lakshmi Sastry, Sheila A. Iverson, Angray S. Kang, Michelle A. Alting-Mees, Dennis R. Burton, Stephen J. Benkovic, Richard Alan Lerner · 发表于：Science · 年份：1989 · DOI：10.1126/science.2531466 · 被引用次数：806 · 研究领域：Monoclonal and Polyclonal Antibodies Research、Glycosylation and Glycoproteins Research、Bacteriophages and microbial interactions

A novel bacteriophage lambda vector system was used to express in Escherichia coli a combinatorial library of Fab fragments of the mouse antibody repertoire. The system allows rapid and easy identification of monoclonal Fab fragments in a form suitable for genetic manipulation. It was possible to generate, in 2 weeks, large numbers of monoclonal Fab fragments against a transition state analog hapten. The methods described may supersede present-day hybridoma technology and facilitate the production of catalytic and other antibodies.