MiR-371-5p Facilitates Pancreatic Cancer Cell Proliferation and Decreases Patient Survival

作者：De He, Huilai Miao, Yumin Xu, Longhui Xiong, Yi Wang, Hongxia Xiang, Heng Zhang, Zhiyong Zhang · 发表于：PLoS ONE · 年份：2014 · DOI：10.1371/journal.pone.0112930 · 被引用次数：47 · 研究领域：MicroRNA in disease regulation、Circular RNAs in diseases、Cancer Mechanisms and Therapy

BACKGROUND: microRNAs (miRNAs) play a critical role in tumorigenesis, either as a tumor suppressor or as an oncogenic miRNA, depending on different tumor types. To date, scientists have obtained a substantial amount of knowledge with regard to miRNAs in pancreatic cancer. However, the expression and function of miR-371-5p in pancreatic cancer has not been clearly elucidated. The aim of this study was to investigate the roles of miR-371-5p in pancreatic cancer and its association with the survival of patients with pancreatic cancer. METHODS: The expression of miR-371-5p was examined in pancreatic duct adenocarcinoma (PDAC) and their adjacent normal pancreatic tissues (ANPT) or in pancreatic cancer cell lines by qRT-PCR. The association of miR-371-5p expression with overall survival was determined. The proliferation and apoptosis of SW-1990 and Panc-1 cells, transfected with miR-371-5p mimics or inhibitor, were assessed using MTT assay and flow cytometry, respectively. The tumorigenicity was evaluated via mice xenograft experiments. miR-371-5p promoter interactions were analyzed by chromatin immunoprecipitation assays (ChIP). Protein expression was analyzed by Western blot. RESULTS: The expression level of miR-371-5p was dramatically upregulated in clinical PDAC tissues compared with ANPT. Patients with high miR-371-5p expression had a significantly shorter survival than those with low miR-371-5p expression. The in vitro and in vivo assays showed that overexpression of miR-371-...