The Structure of the Eukaryotic Ribosome at 3.0 Å Resolution

作者：Adam Ben Shem, Nicolas Garreau de Loubresse, Sergey V. Melnikov, Lasse Bohl Jenner, Gulnara Zh. Yusupova, Marat Yusupov · 发表于：Science · 年份：2011 · DOI：10.1126/science.1212642 · 被引用次数：1154 · 研究领域：RNA and protein synthesis mechanisms、RNA modifications and cancer、Bacterial Genetics and Biotechnology

Ribosomes translate genetic information encoded by messenger RNA into proteins. Many aspects of translation and its regulation are specific to eukaryotes, whose ribosomes are much larger and intricate than their bacterial counterparts. We report the crystal structure of the 80S ribosome from the yeast Saccharomyces cerevisiae--including nearly all ribosomal RNA bases and protein side chains as well as an additional protein, Stm1--at a resolution of 3.0 angstroms. This atomic model reveals the architecture of eukaryote-specific elements and their interaction with the universally conserved core, and describes all eukaryote-specific bridges between the two ribosomal subunits. It forms the structural framework for the design and analysis of experiments that explore the eukaryotic translation apparatus and the evolutionary forces that shaped it.