OBSERVATIONS ON THE EFFECT OF PENICILLIN ON THE REACTION BETWEEN PHAGE AND STAPHYLOCOCCI

作者：A. P. Krueger, Theodore E. Cohn, P. N. Smith, Carolann McGuire · 发表于：The Journal of General Physiology · 年份：1948 · DOI：10.1085/jgp.31.6.477 · 被引用次数：22 · 研究领域：Bacterial Genetics and Biotechnology、Microbial Natural Products and Biosynthesis、Monoclonal and Polyclonal Antibodies Research

The essential facts relating to the reaction between phage, sodium penicillin G, and the K race of Staphylococcus aureus are: 1. Except when [P] is very high, massive lysis of the cellular substrate occurs considerably sooner in the P-PN-B mixture than in preparations containing P alone or PN alone. 2. The accelerative effect is present in concentrations of PN varying from 0.1 to 1 x 10(4) units/ml. 3. Acceleration of lysis can be secured by exposing staphylococci to PN prior to treatment with P. 4. In certain concentrations of P and B in tryptose-phosphate broth, P formation apparently takes place without bacterial reproduction. The extent to which P is produced is influenced very little by [PN](0) but is markedly dependent upon [P](0). With low P/B ratios the [P] curve shows a lag followed by a rapid rise to a peak of 25 to 30 times [P](0). When the P/B ratio approaches unity there is a considerable primary drop in [P] and later an increase which, however, fails to bring the total P produced above [P](0). When P/B is still higher, the [P] curve drops profoundly as the bacteria lyse and never enters into a productive phase. 5. In Locke's solution mixtures of P-PN-B, containing 5 to 10 per cent broth, P formation occurs in the absence of detectable cellular reproduction to the extent of a four- to sixfold increase over [P](0). 6. Direct microscopic examination of wet preparations removed during the P-PN-B reaction has disclosed swelling of the staphylococci. The swollen cells...