MicroRNA‐155 is a novel suppressor of ovarian cancer‐initiating cells that targets CLDN1

作者：Wenxing Qin, Qiusheng Ren, Te Liu, Yongyi Huang, Jiejun Wang · 发表于：FEBS Letters · 年份：2013 · DOI：10.1016/j.febslet.2013.03.023 · 被引用次数：140 · 研究领域：Cancer-related molecular mechanisms research、MicroRNA in disease regulation、Barrier Structure and Function Studies

Previous cDNA microarrays indicated that CLDN1 (claudin-1) is an important gene for ovarian cancer-initiating cell (OCIC) invasion and adhesion. Here, we show that the downregulation of miR-155 in OCICs correlates with CLDN1 overexpression and the suppression of OCIC invasion. Luciferase assays indicate that miR-155 targets CLDN1 mRNA on the 3' UTR. CLDN1 mRNA and claudin-1 protein expression were significantly decreased in miR-155-OCICs. Proliferation assays and Transwell migration assays show that miR-155 significantly suppresses the proliferative and invasive capacity of OCICs. Furthermore, miR-155 suppresses the growth of OCIC xenograft tumors. Thus, overexpression of miR-155 may prevent tumorigenesis in human ovarian cancer through downregulation of CLDN1.