The monoclonal antibody AC1.59 defines a new polymorphic determinant on HLA‐DR molecules

作者：Tiziana Crepaldi, PatriciaMomigliano Richiardi, Fabio Malavasi, Angelo Oscar Carbonara, M. Igarashi, Soldano Ferrone · 发表于：Tissue Antigens · 年份：1985 · DOI：10.1111/j.1399-0039.1985.tb00931.x · 被引用次数：11 · 研究领域：Monoclonal and Polyclonal Antibodies Research、T-cell and B-cell Immunology、Immune Cell Function and Interaction

2-dimensional gel electrophoresis analysis and sequential immunoprecipitation studies have shown that the monoclonal antibody (MoAb) AC1.59 recognizes a gene product of the HLA-DR locus. The determinant defined by the MoAb AC-1.59 has an unusual distribution on HLA-DR allospecificities, since it is expressed by HLA-DR1, DRw8, DRw9 antigens and by subtypes of HLA-DRw6 and HLA-DR4 antigens. In the latter allospecificity the expression of the MoAb AC1.59 defined determinant correlates with the cellularly defined HLA-Dw4 alloantigen. The present investigation provides evidence of serological heterogeneity within HLA-DR alloantigens. Furthermore these results suggest that monoclonal antibodies can sharpen the discriminatory power of serological assays to dissect the heterogeneity and complexity of HLA-DR alloantigens.