PCR‐RFLP and AP‐PCR of rbc L and ITS of rDNA Show That × Taxodiomeria peizhongii ( Taxodium × Cryptomeria ) Is not an Intergeneric Hybrid

作者：Yan Ling, Weifeng Lü, Fan Lü, Yu‐Guo Wang, Jiakuan Chen, Wenju Zhang · 发表于：Journal of Integrative Plant Biology · 年份：2006 · DOI：10.1111/j.1744-7909.2006.00209.x · 被引用次数：6 · 研究领域：Plant Disease Resistance and Genetics、Genomics and Phylogenetic Studies、Plant and Fungal Interactions Research

Abstract × Taxodiomeria peizhongii Z. J. Ye, J. J. Zhang et S. H. Pan was regarded as a new intergeneric hybrid between Taxodium mucronatum Tenore (as the female donor) and Cryptomeria fortunei Hooibrenk ex Otto et Dietr (as the male donor). To confirm the authenticity of the intergeneric hybrid, we analyzed the rbc L gene and the internal transcribed spacer (ITS) of 26S‐18S ribosomal RNA gene of the three species using polymerase chain reaction‐restriction fragment length polymorphism (PCR‐RFLP) and arbitrarily primed PCR (AP‐PCR), and obtained the following results: i) Taxodiomeria peizhongii had the same RFLP maps of the rbc L gene and the ITS as Taxodium mucronatum , but was different from C. fortune i; ii) a 311‐bp PCR amplification product was obtained in C. fortunei by AP‐PCR of ITS, but was not found in Taxodiomeria peizhongii. Our results have demonstrated that C. fortunei did not provide any genome for Taxodiomeria peizhongii , implying that T. peizhongii is not an intergeneric hybrid between the two species. (Managing editor: Wei Wang)