Comparison of Three Commercially Available Dengue NS1 Antigen Capture Assays for Acute Diagnosis of Dengue in Brazil

作者：Monique da Rocha Queiroz Lima, Rita Maria Ribeiro Nogueira, Hermann Gonçalves Schatzmayr, Flávia Barreto dos Santos · 发表于：PLoS neglected tropical diseases · 年份：2010 · DOI：10.1371/journal.pntd.0000738 · 被引用次数：146 · 研究领域：Mosquito-borne diseases and control、Viral Infections and Outbreaks Research、Biological Research and Disease Studies

BACKGROUND: Dengue is associated with explosive urban epidemics and has become a major public health problem in many tropical developing countries, including Brazil. The laboratory diagnosis of dengue can be carried out using several approaches, however sensitive and specific assays useful to diagnose in the early stage of fever are desirable. The flavivirus non-structural protein NS1, a highly conserved and secreted glycoprotein, is a candidate protein for rapid diagnosis of dengue in endemic countries. METHODOLOGY/PRINCIPAL FINDINGS: We aimed to evaluate the potential use of 3 commercial kits in a panel of 450 serum samples for early diagnosis of dengue in Brazil. The PanBio Early ELISA (PanBio Diagnostics) showed a sensitivity of 72.3% (159/220) and a specificity of 100%, while the sensitivity of the Platelia NS1 assay (Biorad Laboratories) was 83.6% (184/220). However, the highest sensitivity (89.6%; 197/220) was obtained by using the NS1 Ag Strip (Biorad Laboratories). A lower sensitivity was observed in DENV-3 cases by all 3 kits. Serum positive by virus isolation were more often positive than cases positive by RT-PCR by all three assays and a higher detection rate was observed during the first four days after the onset of the symptoms. The presence or absence of IgM showed no influence in the confirmation by the pan-E Early ELISA (P = 0,6159). However, a higher confirmation by both Platelia NS1 (Biorad) and Dengue NS1 Ag Strip (Biorad) in the absence of IgM was statist...