Radioimmunoassay for Desmosine

作者：Graham S. King, V. S. Mohan, BARRY C. STARCHER · 发表于：Connective Tissue Research · 年份：1980 · DOI：10.3109/03008208009152362 · 被引用次数：103 · 研究领域：Connective tissue disorders research、Proteoglycans and glycosaminoglycans research、Peptidase Inhibition and Analysis

A radioimmunoassay was developed for the determination of desmosine. Desmosine conjugated to albumin was injected into rabbits which developed useful titers of serum antibodies after six months. A radioactive probe was prepared with desmosine using [125I]-Bolton-Hunter reagent. Bound desmosine was separated from free desmosine by cellulose acetate filter binding. The sensitivity of the assay is 1-50 picomoles of desmosine. The antibody is highly selective for desmosine, reacting less than 1% with other known crosslinks. Some prepurification may be necessary with complex samples which contain trace amounts of elastin peptides.