Enkephalin inhibits release of substance P from sensory neurons in culture and decreases action potential duration.

作者：Anne W. Mudge, Susan E. Leeman, G D Fischbach · 发表于：Proceedings of the National Academy of Sciences · 年份：1979 · DOI：10.1073/pnas.76.1.526 · 被引用次数：549 · 研究领域：Neuroscience and Neural Engineering、Neurobiology and Insect Physiology Research、Olfactory and Sensory Function Studies

Sensory neurons grown in dispersed cell culture in the absence of non-neuronal cell types contain immunoreactive substance P that is chemically similar to synthetic substance P. When depolarized in high-K+ media (30-120 mM), the neurons release this peptide by a Ca2+-dependent mechanism. An enkephalin analogue, [D-Ala2]enkephalin amide, at 10 micron inhibits the K+-evoked release of substance P. At the same or lower concentrations, [D-Ala2]enkephalin amide and enkephalin decrease the duration of the Ca2+ action potential evoked and recorded in dorsal root ganglion cell bodies without affecting the resting membrane potential or resting membrane conductance. This modulation of voltage-sensitive channels may account for the inhibition of substance P release.