Nodal activity in the node governs left-right asymmetry

作者：Jane Brennan, Dominic P. Norris, Elizabeth J. Robertson · 发表于：Genes & Development · 年份：2002 · DOI：10.1101/gad.1016202 · 被引用次数：299 · 研究领域：Developmental Biology and Gene Regulation、Congenital heart defects research、Wnt/β-catenin signaling in development and cancer

Nodal is expressed at the lateral edges of the mouse node, but its function in this "organizer" tissue remains unknown due to the early lethality of Nodal mutant embryos. Here we used a genetic strategy to selectively remove Nodal activity from the node. Embryos lacking Nodal in the node fail to initiate molecular asymmetry in the left lateral plate mesoderm and exhibit multiple left-right patterning defects. Nodal may also act as a short-range signal to establish a functional midline barrier. Our findings confirm that the mouse node is instrumental in initiating left-right axis specification and identify Nodal as the key morphogen regulating this process.