Impact of Estradiol Benzoate and Progesterone on the Activity of Carbonic Anhydrase of Erythrocytes in Ducks (Anatis domesticae Caro)

作者：Yanyou Wu, Xiangcheng Zhao, P. P. Li, Yanyou Wu · 发表于：International Conference on Bioinformatics and Biomedical Engineering · 年份：2010 · DOI：10.1109/icbbe.2010.5518031 · 研究领域：Hormonal and reproductive studies、Reproductive biology and impacts on aquatic species、Neuroendocrine regulation and behavior

The impact of estradiol benzoate and progesterone on the duck erythrocyte carbonic anhydrase (CA) activity of in vitro culture was studied. The impact of estradiol benzoate and progesterone on CA activity of in vitro culture duck erythrocytes was significant and varied with the incubation durations. When the duck erythrocytes were incubated 2, 14 hours, estradiol benzoate increased the CA activity. When the duck erythrocytes were incubated thirty hours, estradiol benzoate at a higher addition (more than 200 pg ml-1) had no facilitation to the CA activity. Progesterone with addition in the range of 0.5 to 50 ng ml-1 promoted the CA activity when the duck erythrocytes were incubated fourteen hours. The CA activity was also decreased by progesterone when the duck erythrocytes were incubated two hours in MEM medium containing progesterone at the addition of 1 to 10 ng ml-1. Progesterone at the addition of 0.5 ng ml-1 inhibited the CA activity when the duck erythrocytes were incubated thirty hours. These findings can help to understand the interaction between CA and reproductive hormones, and account for the impact of exogenous hormones administration or endogenous estrogens analogs inhalation on the eggshell formation of right thickness and some other biological functions.