Isolation and Identification of Acaricidal Compounds in Inula japonica (Asteraceae)

作者：Dandan Duan, Chunya Bu, J. A. Cheng, Y. N. Wang, Guanglu Shi · 发表于：Journal of Economic Entomology · 年份：2011 · DOI：10.1603/ec10129 · 被引用次数：16 · 研究领域：Insect Pest Control Strategies、Insect-Plant Interactions and Control、Insect Pheromone Research and Control

We identified acaricidal compounds in Inula japonica Thunberg (Asteraceae) that were effective against carmine spider mite, Tetranychus cinnabarinus (Boisduval). Petroleum-ether extracts from I. japonica were toxic to T. cinnabarinus, with a 50% lethal concentration (LC50) value of 1.18 mg/ml. Silica gel column chromatography was used to separate the acaricidal components. Seventeen of 38 fraction groups had mite mortality rates >50%, nine fraction groups had rates >60%, and three fraction groups had rates >80% at 2 mg/ml. The major volatile compounds in the bioactive fraction groups were identified by gas chromatography-mass spectroscopy, and beta-sitosterol (1), stigmasterol (2), lupeol (3), and alpha-amyrin (4) were identified and determined to have acaricidal activity against T. cinnabarinus in vitro.