Icariin attenuates hypoxia‐induced oxidative stress and apoptosis in osteoblasts and preserves their osteogenic differentiation potential in vitro

作者：Hui-Lan Ma, Xiaofei Ma, B.‐F. Ge, Ping Zhen, Jian Zhou, Ying Gao, Cory J. Xian, Keming Chen · 发表于：Cell Proliferation · 年份：2014 · DOI：10.1111/cpr.12147 · 被引用次数：65 · 研究领域：Medicinal Plant Pharmacodynamics Research、Bone Metabolism and Diseases、Osteoarthritis Treatment and Mechanisms

OBJECTIVES: Icariin, a prenylated flavonol glycoside isolated from traditional Chinese medicinal herb of the genus Epimedium, has been demonstrated to be a potential alternative therapy for osteoporosis, and its action mechanism so far has been mainly attributed to its phytoestrogenic property. As blood supply to bone is considerably reduced with ageing and by the menopause, we hypothesized that icariin treatment would reduce bone loss by preventing ischaemia-induced hypoxic damages to bone. MATERIALS AND METHODS: To investigate effects of icariin treatment on cultured rat calvarial osteoblasts exposed to hypoxic conditions (2% oxygen). RESULTS: Compared to normoxic control, cell viability decreased with time to 50% by 48 h in the hypoxic group, and icariin attenuated the reduction, dose dependently, with 10(-6) and 10(-5) m concentrations showing significant protective effects. Icariin also inhibited increase of lactate dehydrogenase activity in culture media. Measurements on oxidative stress, cell cycling and cell survival indicated that icariin protected osteoblasts by reducing production of reactive oxygen species and malondialdehyde, increasing superoxide dismutase activity, arresting the cell cycle and inhibiting apoptosis. Icariin also preserved osteogenic differentiation potential of the hypoxic cells in a dose-dependent manner, compared to the hypoxia alone group, as revealed by increased levels of RUNX-2, OSX and BMP-2 gene expression, alkaline phosphatase activity,...