Absence of a Low‐Molecular‐Weight DNA Polymerase from Nuclei of the Yeast, Saccharomyces cerevisiae

作者：Ulrike Wintersberger · 发表于：European Journal of Biochemistry · 年份：1974 · DOI：10.1111/j.1432-1033.1974.tb03888.x · 被引用次数：43 · 研究领域：DNA Repair Mechanisms、DNA and Nucleic Acid Chemistry、RNA and protein synthesis mechanisms

The DNA polymerase activity associated with purified nuclei of the yeast, Saccharomyces cerevisiae, was analyzed by sucrose gradient centrifugation. Using different salt concentrations there was no polymerase activity found sedimenting in the 3–4‐S region. On the contrary, the nuclear enzyme shows a main peak around 8 S, similar to the DNA polymerases from yeast cytoplasm. Other properties tested (e.g. behaviour on ion‐exchange columns, dependence of activity on salt concentration, inhibition by p‐chloromercuribenzoate etc.) were also found identical with those of the cytoplasmic DNA polymerases.