The pentose shunt in wild‐type and glucose‐6‐phosphate dehydrogenase deficient Drosophila melanogaster

作者：B. W. Geer, James T. Bowman, John R. Simmons · 发表于：Journal of Experimental Zoology · 年份：1974 · DOI：10.1002/jez.1401870110 · 被引用次数：32 · 研究领域：Ion Transport and Channel Regulation、Metabolism and Genetic Disorders、Mitochondrial Function and Pathology

Abstract Four‐day old Drosophila melanogaster homozygous or hemizygous for the glucose‐6‐phosphate dehydrogenase mutant allele, Zw−, possess slightly less than 10% as much glucose‐6‐phosphate dehydrogenase activity in whole‐fly homogenates as their wild‐type, Zw+, counterparts. The reduction of glucose‐6‐phosphate dehydrogenase activity in Zw− adults had no effect upon 6‐phosphogluconate dehydrogenase, phosphofructokinase, or malic enzyme activities. However, activities of the non‐oxidative pentose shunt enzymes were significantly greater in Zw− adults than in Zw+ adults. Radiotracer procedures indicate that the oxidative phase of the pentose shunt is blocked in Zw− adults. Zw− larvae grow less well on a defined minimal amino acid diet lacking fatty acids and whole nucleic acid than do Zw+ larvae, indicating that Zw− individuals have a lower biosynthetic capacity than Zw+ individuals.