Transforming growth factor-beta 1 stimulates chondrogenesis and inhibits osteogenesis in high density culture of periosteum-derived cells.

作者：Motoki Iwasaki, Kiyoi Nakata, Hirokazu Nakahara, Takanobu Nakase, Tomonori Kimura, Koji Kimata, Arnold I. Caplan, Katsuhiko Ono · 发表于：Endocrinology · 年份：1993 · DOI：10.1210/endo.132.4.8462458 · 被引用次数：146 · 研究领域：Osteoarthritis Treatment and Mechanisms、Mesenchymal stem cell research、Periodontal Regeneration and Treatments

Chick periosteum-derived mesenchymal cells have been reported to exhibit both osteogenic and chondrogenic potentials in high cell density culture conditions. Using this culture system, the effects of transforming growth factor-beta (TGF beta) on proliferation and differentiation of periosteal mesenchymal cells were studied. Supplementation with TGF beta 1 at doses of 0.3-1.0 ng/ml shortened the time course of chondrogenesis and increased the amount of cartilage formed in the lower part of the culture. On the other hand, the amount of bone formed in the upper part of the culture decreased with TGF beta treatment, whereas the time course of osteogenesis remained unaffected.