High Efficiency Transformation of Cultured Tobacco Cells

作者：Gynheung An · 发表于：PLANT PHYSIOLOGY · 年份：1985 · DOI：10.1104/pp.79.2.568 · 被引用次数：461 · 研究领域：Plant tissue culture and regeneration、Transgenic Plants and Applications、Plant Virus Research Studies

Tobacco calli were transformed at levels up to 50% by cocultivation of tobacco cultured cells with Agrobacterium tumefaciens harboring the binary transfer-DNA vector, pGA472, containing a kanamycin resistance marker. Transformation frequency was dependent on the physiological state of the tobacco cells, the nature of Agrobacterium strain and, less so, on the expression of the vir genes of the tumor-inducing plasmid. Maximum transformation frequency was obtained with exponentially growing plant cells, suggesting that rapid growth of plant cells is an essental factor for efficient transformation of higher plants.