Improved silver staining protocols for high sensitivity protein identification using matrix-assisted laser desorption/ionization-time of flight analysis

作者：Ejvind Mørtz, Thomas Nylandsted Krogh, Henrik Vorum, Angelika Görg · 发表于：PROTEOMICS · 年份：2001 · DOI：10.1002/1615-9861(200111)1:11<1359::aid-prot1359>3.0.co;2-q · 被引用次数：507 · 研究领域：Advanced Proteomics Techniques and Applications、Mass Spectrometry Techniques and Applications、Advanced Biosensing Techniques and Applications

In proteomics it is essential to be able to detect proteins separated by gel electrophoresis at high sensitivity. Silver staining is currently the most popular method. Here we present silver staining protocols that are optimized for staining sensitivity, peptide recovery and compatibility with digestion and mass spectrometry.