Note: Identification ofLactobacillus delbrueckiisubspeciesbulgaricusand subspecieslactisdairy isolates by amplified rDNA restriction analysis

作者：Giorgio Giraffa, P. De Vecchi, Lia Rossetti · 发表于：Journal of Applied Microbiology · 年份：1998 · DOI：10.1046/j.1365-2672.1998.00606.x · 被引用次数：72 · 研究领域：Probiotics and Fermented Foods、Genomics and Phylogenetic Studies、Bacteriophages and microbial interactions

Amplification and restriction analysis of the 16S rRNA gene (PCR-ARDRA) was used to identify Lactobacillus delbrueckii dairy isolates and to assign them to its three subspecies (delbrueckii, lactis and bulgaricus). PCR-ARDRA allowed confirmation of predictions from the DNA database regarding the restriction mapping of the 16S sequence to be verified in practice. PCR-ARDRA was shown to be a reliable and rapid method for identifying Lact. delbrueckii isolates at the subspecies level and for differentiating this species from Lact. helveticus and Lact. acidophilus.