Selective in Vitro Growth of T Lymphocytes from Normal Human Bone Marrows

作者：Doris A. Morgan, Francis W. Ruscetti, Robert C. Gallo · 发表于：Science · 年份：1976 · DOI：10.1126/science.181845 · 被引用次数：2501 · 研究领域：Viral-associated cancers and disorders、T-cell and Retrovirus Studies、Immune Cell Function and Interaction

Selective growth of T lymphocytes occurred when unfractionated normal human bone marrow cells were cultured with conditioned medium obtained from phytohemagglutinin-stimulated normal human lymphocytes (Ly-CM). Cultures of up to 90 percent T cells have been maintained for more than 9 months. The T cells exhibited a strict growth dependence upon Ly-CM and were consistently negative for Epstein-Barr viral information.