Molecular cloning and sequence analysis of cDNA encoding human cholesterol 7α‐hydroxylase

作者：Mitsuhide Noshiro, Kyuichiro Okuda · 发表于：FEBS Letters · 年份：1990 · DOI：10.1016/0014-5793(90)80992-r · 被引用次数：111 · 研究领域：Hormonal and reproductive studies、Steroid Chemistry and Biochemistry、Cholesterol and Lipid Metabolism

A complete cDNA clone encoding human cholesterol 7 alpha-hydroxylase has been isolated using a rat P-450ch7 alpha cDNA insert [(1989) FEBS Lett. 257, 97-100] as a probe and totally sequenced. The cDNA contained 1512-base pair open reading frame encoding 504 amino acid residues (Mr 57,630), 39-base pair 5'-untranslated region 1322-base pair 3'-ultranslated region including 20 nucleotides of poly A tail in the total length of 2873 base pairs. The deduced amino acid sequence showed 82% similarity to rat P-450ch7 alpha. Unique amino acid residues were observed in putative binding domains for heme and steroid which are highly conserved in most steroidogenic P-450s.