Transferred NOE analyses of conformations of peptides as bound to membrane bilayer of phospholipid; mastoparan‐X

作者：Kaori Wakamatsu, Tsutomu Higashijima, Masahiko Fujino, Terumi Nakajima, Tatsuo Miyazawa · 发表于：FEBS Letters · 年份：1983 · DOI：10.1016/0014-5793(83)81062-x · 被引用次数：66 · 研究领域：Healthcare and Venom Research、Venomous Animal Envenomation and Studies、Lipid Membrane Structure and Behavior

The 270‐MHz 1H‐NMR spectra of mastoparan‐X, a tetradecapeptide toxin from hornet venom, have been observed in the presence of vesicles of perdeuterated dilauroylphosphatidylcholine ([2H64]DLPC). By the analysis of transferred nuclear Overhauser effects (TRNOE), the mastoparan‐X molecule as bound to [2H64]DLPC vesicles is found to take an α‐helical form in the C‐terminal (and central) part. The TRNOE analysis with perdeuterated phospholipid is a unique method for the elucidation of conformation of peptide (and drug) molecules as bound to phospholipid membranes.