Electron microscopy of chromosomal changes in ambystomal somatic cells during the mitotic cycle

作者：William M. Bloom · 发表于：The Anatomical Record · 年份：1970 · DOI：10.1002/ar.1091670302 · 被引用次数：6 · 研究领域：Microtubule and mitosis dynamics、Nuclear Structure and Function

Abstract This sequence of electron micrographs shows the progressive changes undergone by the dispersed chromosomal fibrils of the interphase nucleus as they begin to condense, in early prophase, into clumps centered on three types of small nuclear structures. These bodies do not seem to have been described previously in detail in connection with mitosis, although they were mentioned as a group in a preliminary note by Bloom (1963). In the present report they are divided into three groups indicated as F1, F2 and F3 and are labelled 1, 2 or 3 in the accompanying electron micrographs. The clumps enlarge by continued accretion of more and more fibrils concentrated about these three types of bodies until the chromosomes of metaphase are formed. The inference that the bodies depicted here form focal points for the condensation of dispersed chromosomal filaments rests solely on the repeated finding of the progressive aggregation of the fibrils about them. Further studies are in progress on factors mediating these changes and on the nature of the F bodies.