Validation of candidate gene markers for marker-assisted selection of potato cultivars with improved tuber quality

作者：Li Li, Eckhard Tacke, Hans-Reinhardt Hofferbert, Jens Lübeck, Josef Strahwald, Astrid M. Draffehn, Birgit Walkemeier, Christiane Gebhardt · 发表于：Theoretical and Applied Genetics · 年份：2013 · DOI：10.1007/s00122-012-2035-z · 被引用次数：84 · 研究领域：Plant Pathogens and Resistance、Potato Plant Research、Plant Disease Resistance and Genetics

Tuber yield, starch content, starch yield and chip color are complex traits that are important for industrial uses and food processing of potato. Chip color depends on the quantity of reducing sugars glucose and fructose in the tubers, which are generated by starch degradation. Reducing sugars accumulate when tubers are stored at low temperatures. Early and efficient selection of cultivars with superior yield, starch yield and chip color is hampered by the fact that reliable phenotypic selection requires multiple year and location trials. Application of DNA-based markers early in the breeding cycle, which are diagnostic for superior alleles of genes that control natural variation of tuber quality, will reduce the number of clones to be evaluated in field trials. Association mapping using genes functional in carbohydrate metabolism as markers has discovered alleles of invertases and starch phosphorylases that are associated with tuber quality traits. Here, we report on new DNA variants at loci encoding ADP-glucose pyrophosphorylase and the invertase Pain-1, which are associated with positive or negative effect with chip color, tuber starch content and starch yield. Marker-assisted selection (MAS) and marker validation were performed in tetraploid breeding populations, using various combinations of 11 allele-specific markers associated with tuber quality traits. To facilitate MAS, user-friendly PCR assays were developed for specific candidate gene alleles. In a multi-parental p...