Serum metabolomics reveals pathways and biomarkers associated with asthma pathogenesis

作者：Jeeyoun Jung, Seung‐Hyun Kim, H.‐S. Lee, Gil‐Soon Choi, Young‐Sang Jung, Do Hyun Ryu, Hae‐Sim Park, Geum‐Sook Hwang · 发表于：Clinical & Experimental Allergy · 年份：2013 · DOI：10.1111/cea.12089 · 被引用次数：168 · 研究领域：Metabolomics and Mass Spectrometry Studies、Asthma and respiratory diseases、Advanced Chemical Sensor Technologies

BACKGROUND: Asthma is a chronic inflammatory disease caused by complex interactions of genetic, epigenetic, and environmental factors. For this reason, new approaches are required to clarify the pathogenesis of asthma by systemic review. OBJECTIVE: We applied a (1)H-NMR metabolomics approach to investigate the altered metabolic pattern in sera from patients with asthma and sought to identify the mechanism underlying asthma and potential biomarkers. METHOD: A global profile of sera from patients with asthma (n = 39) and controls (n = 26) was generated using (1)H-NMR spectroscopy coupled with multivariate statistical analysis. Endogenous metabolites in serum were rapidly measured using the target-profiling procedure. RESULTS: Multivariate statistical analysis showed a clear distinction between patients with asthma and healthy subjects. Sera of asthma patients were characterized by increased levels of methionine, glutamine, and histidine and by decreased levels of formate, methanol, acetate, choline, O-phosphocholine, arginine, and glucose. The metabolites detected in the sera of patients with asthma are involved in hypermethylation, response to hypoxia, and immune reaction. Furthermore, the levels of serum metabolites from patients with asthma correlated with asthma severity; in particular, lipid metabolism was altered in patients with lower forced expiratory volume in 1 s percentage (FEV(1)%) predicted values. In addition, potential biomarkers showed strong predictive power in...