cDNA cloning and sequencing of component C9 of proteasomes from rat hepatoma cells

作者：Atsushi Kumatori, K. Tanaka, Tomohiro Tamura, Tsutomu Fujiwara, Akira Ichihara, Fuminori Tokunaga, Aya Onikura, Sadaaki Iwanaga · 发表于：FEBS Letters · 年份：1990 · DOI：10.1016/0014-5793(90)80267-m · 被引用次数：67 · 研究领域：Ubiquitin and proteasome pathways、Peptidase Inhibition and Analysis、Protease and Inhibitor Mechanisms

The nucleotide sequence of component C9 of rat proteasomes (multicatalytic proteinase complexes) has been determined from a recombinant cDNA clone isolated by screening a Reuber H4TG hepatoma cell cDNA library using synthetic oligodeoxynucleotide probes corresponding to partial amino acid sequences of the protein. The predicted sequence of C9 consists of 261 amino acid residues with a calculated molecular weight of 29,496. The C9 component is a novel protein, differing from known proteins, but its primary structure resembles those of other proteasome components, including C2, C3 and C5, although its similarity to C5 is relatively low, suggesting that proteasomes consist of a family of proteins that have evolved from a common ancestor.