Photoinactivation of Catalase Occurs under Both High- and Low-Temperature Stress Conditions and Accompanies Photoinhibition of Photosystem II

作者：J. Feierabend, Cornelia Schaan, Birgit Hertwig · 发表于：PLANT PHYSIOLOGY · 年份：1992 · DOI：10.1104/pp.100.3.1554 · 被引用次数：198 · 研究领域：Plant Stress Responses and Tolerance、Photosynthetic Processes and Mechanisms、Plant responses to elevated CO2

Severe photoinactivation of catalase (EC 1.11.1.6) and a decline of variable fluorescence (F(v)), indicating photoinhibition of photosynthesis, were observed as rapid and specific symptoms in leaves exposed to a high heat-shock temperature of 40 degrees C as well as in leaves exposed to low chilling temperatures in white light of only moderately high photosynthetic photon flux density of 520 muE m(-2) s(-1). Other parameters, such as peroxidase (EC 1.11.1.7), glycolate oxidase (EC 1.1.3.1), glutathione reductase (EC 1.6.4.2), or the chlorophyll content, were hardly affected under these conditions. At a compatible temperature of 22 degrees C, the applied light intensity did not induce severe photoinactivations. In darkness, exposures to high or low temperatures did not affect catalase levels. Also, decline of F(v) in light was not related to temperature sensitivity in darkness. The effective low-temperature ranges inducing photoinactivation of catalase differed significantly for chilling-tolerant and chilling-sensitive plants. In leaves of rye (Secale cereale L.) and pea (Pisum sativum L.), photoinactivation occurred only below 15 degrees C, whereas inactivation occurred at 15 degrees C in cucumber (Cucumis sativus L.) and maize (Zea mays L.). The behavior of F(v) was similar, but the difference between chilling-sensitive and chilling-tolerant plants was less striking. Whereas the catalase polypeptide, although photoinactivated, was not cleaved at 0 to 4 degrees C, the D1 prot...