Tris(hydroxymethyl)aminomethane (C4H11NO3) Induced a Ripple Phase in Supported Unilamellar Phospholipid Bilayers

作者：Jianxun Mou, Jie Yang, Zhifeng Shao · 发表于：Biochemistry · 年份：1994 · DOI：10.1021/bi00181a001 · 被引用次数：107 · 研究领域：Lipid Membrane Structure and Behavior、Protein Structure and Dynamics、Hemoglobin structure and function

A commonly used buffer compound, tris(hydroxymethyl)aminomethane (C4H11NO3), was found to induce a ripple phase in supported unilamellar phospholipid bilayers at room temperature. The ripple structure showed various types of domains that could extend to several micrometers in length with many well-defined bendings of either 120 degrees or 60 degrees. Two different periodic ripples were found to coexist in 1,2-dipentadecanoyl-sn-glycero-3-phosphatidylcholine bilayers. Some intermediate states during the transition to the ripple phase were also observed.