Fibroblasts Transformed with v- src Show Enhanced Formation of an Inositol Tetrakisphosphate

作者：R. M. Johnson, William J. Wasilenko, R.R. Mattingly, M Weber, J. C. Garrison · 发表于：Science · 年份：1989 · DOI：10.1126/science.2506643 · 被引用次数：66 · 研究领域：Pancreatitis Pathology and Treatment、Protein Kinase Regulation and GTPase Signaling、Wnt/β-catenin signaling in development and cancer

The tyrosine kinase pp60v-src, encoded by the v-src oncogene, seems to regulate phosphatidylinositol metabolism. The effect of pp60v-src on control points in inositol phosphate production was examined by measuring the amounts of inositol polyphosphates in Rat-1 cells expressing wild-type or mutant forms of the protein. Expression of v-src-resulted in a five- to sevenfold elevation in the steady-state amount of an isomer of inositol tetrakisphosphate, whereas the concentrations of inositol trisphosphates or other inositol tetrakisphosphates were not affected. The activity of a key enzyme in the formation of inositol tetrakisphosphates, inositol (1,4,5)-trisphosphate 3-kinase, was increased six- to eightfold in cytosolic extracts prepared from the v-src-transformed cells, suggesting that this enzyme may be one target for the pp60v-src kinase and that it may participate in the synthesis of novel, higher order inositol phosphates.