The diploid genome sequence of Candida albicans

作者：Ted Jones, Nancy A. Federspiel, Hiroji Chibana, Jan M. Dungan, Sue Kalman, B B Magee, George R. Newport, Yvonne R. Thorstenson, Nina Agabian, P. T. Magee, Ronald W. Davis, Stewart Scherer · 发表于：Proceedings of the National Academy of Sciences · 年份：2004 · DOI：10.1073/pnas.0401648101 · 被引用次数：782 · 研究领域：Antifungal resistance and susceptibility、Plant Pathogens and Fungal Diseases、Fungal Infections and Studies

We present the diploid genome sequence of the fungal pathogen Candida albicans. Because C. albicans has no known haploid or homozygous form, sequencing was performed as a whole-genome shotgun of the heterozygous diploid genome in strain SC5314, a clinical isolate that is the parent of strains widely used for molecular analysis. We developed computational methods to assemble a diploid genome sequence in good agreement with available physical mapping data. We provide a whole-genome description of heterozygosity in the organism. Comparative genomic analyses provide important clues about the evolution of the species and its mechanisms of pathogenesis.