FORMATION AND DETECTION OF RNA-DNA HYBRID MOLECULES IN CYTOLOGICAL PREPARATIONS

作者：Joseph G. Gall, Mary Lou Pardue · 发表于：Proceedings of the National Academy of Sciences · 年份：1969 · DOI：10.1073/pnas.63.2.378 · 被引用次数：1275 · 研究领域：DNA and Nucleic Acid Chemistry、Plant Genetic and Mutation Studies

A technique is described for forming molecular hybrids between RNA in solution and the DNA of intact cytological preparations. Cells in a conventional tissue squash are immobilized under a thin layer of agar. Next they are treated with alkali to denature the DNA and then incubated with tritium-labeled RNA. The hybrids are detected by autoradiography. The technique is illustrated by the hybridization of ribosomal RNA to the amplified ribosomal genes in oocytes of the toad Xenopus. A low level of gene amplification was also detected in premeiotic nuclei (oogonia).