PROSTAGLANDIN PRODUCTION BY CALVARIAE FROM SHAM OPERATED AND OOPHORECTOMIZED RATS: EFFECT OF 17β-ESTRADIOL IN VIVO

作者：Jean H.M. Feyen, Lawrence G. Raisz · 发表于：Endocrinology · 年份：1987 · DOI：10.1210/endo-121-2-819 · 被引用次数：74 · 研究领域：Estrogen and related hormone effects、Inflammatory mediators and NSAID effects、Bone health and osteoporosis research

The mechanism by which estrogen inhibits bone resorption in vivo is not known. Since prostaglandin E2 (PGE2) is a potent stimulator of bone resorption in vitro, we tested the hypothesis that estrogens might affect bone indirectly by inhibiting endogenous PGE2 synthesis. Cultured parietal bones from 7- to 9-week-old rats showed a gradual release of immunoreactive PGE2 in vitro. Oophorectomy resulted in a two-fold increase in PGE2 release. Treatment in vivo with low doses of 17 beta-estradiol or high doses of 17 alpha-estradiol 26 h and 2 h prior to sacrifice inhibited bone PGE2 release in vitro. Addition of 17 beta-estradiol to calvariae in vitro did not affect PGE2 release, whereas cortisol inhibited PGE2 release.