Inactivation and proteolytic cleavage of phage lambda repressor in vitro in an ATP-dependent reaction.

作者：Jeffrey W. Roberts, Charles W.M. Roberts, David W. Mount · 发表于：Proceedings of the National Academy of Sciences · 年份：1977 · DOI：10.1073/pnas.74.6.2283 · 被引用次数：121 · 研究领域：Bacteriophages and microbial interactions、Cancer Research and Treatments、Bacterial Genetics and Biotechnology

We have reproduced in vitro the inactivation of bacteriophage lambda repressor that occurs in vivo when a lambda lysogen is treated with agents such as ultraviolet radiation that attack DNA. ATP and a divalent cation are required for the inactivation reaction. The ind- repressor is insensitive to the inactivation mechanism. A proteolytic cleavage of repressor accompanies inactivation in vitro, as it does in vivo.