Supported planar membranes in studies of cell-cell recognition in the immune system

作者：Harden M. McConnell, Tania H. Watts, R. Weis, Adrienne A. Brian · 发表于：Biochimica et Biophysica Acta (BBA) - Reviews on Biomembranes · 年份：1986 · DOI：10.1016/0304-4157(86)90016-x · 被引用次数：561 · 研究领域：Lipid Membrane Structure and Behavior、Force Microscopy Techniques and Applications、Nanopore and Nanochannel Transport Studies

The fusion between two lipid membranes is a ubiquitous mechanism in cell traffic and pathogens invasion. Yet it is not well understood how two distinct bilayers overcome the energy barriers towards fusion and reorganize themselves to form a unique continuous bilayer. The magnitudes and numbers of these energy barriers are themselves an open question. To tackle these issues, we developed a new tool that allows to control the forces applied between two supported lipid bilayers (SLBs) deposited on superparamagnetic beads. By applying a magnetic field, the beads self-organize along field lines in chains of beads and compress the two membranes on the contact zone. Using the diffusion of fluorescently labelled lipids from one bilayer to the other allows us to identify fusion of the bilayers in contact. We applied increasing forces on SLBs and increased the occurrence of fusion. This experimental system allows the simultaneous study of tens of facing bilayers in a single experiment and mitigates the stochasticity of the fusion process. It is thus a powerful tool to test the various parameters involved in the membrane fusion process.