Direct evidence of nitric oxide production from bovine aortic endothelial cells using new fluorescence indicators: diaminofluoresceins

作者：Naoki Nakatsubo, Hirotatsu Kojima, Kazuya Kikuchi, Hiroshi Nagoshi, Yasunobu Hirata, Daisuke Maeda, Yasuyuki Imai, Tatsuro Irimura, Tetsuo Nagano · 发表于：FEBS Letters · 年份：1998 · DOI：10.1016/s0014-5793(98)00440-2 · 被引用次数：394 · 研究领域：Nitric Oxide and Endothelin Effects、Analytical Chemistry and Sensors、Electrochemical sensors and biosensors

The measurement of nitric oxide (NO) is important for direct examination of the regulatory roles of NO in various biological systems. Diaminofluoresceins (DAFs), new fluorescence indicators for NO, were applied to detect the release of NO from bovine aortic endothelial cells (ECs). DAFs react with NO to yield the corresponding green-fluorescent triazolofluoresceins, which provide the advantages of specificity, sensitivity and a simple protocol for the direct detection of NO. Using these DAFs, we could detect the generation of NO not only from inducible NO synthase expressed in macrophages, but also from constitutive NO synthase expressed in ECs.