Strain improvement for enhanced production of cellulase in Trichoderma viride

作者：Fei Xu, Jiao Wang, Shiju Chen, Wensheng Qin, Yu Zhang, Hongxin Zhao, Xin‐Hui Xing, He‐Ping Li · 发表于：Applied Biochemistry and Microbiology · 年份：2011 · DOI：10.1134/s0003683811010212 · 被引用次数：45 · 研究领域：Biofuel production and bioconversion、Microbial Metabolites in Food Biotechnology、Microbial Metabolic Engineering and Bioproduction

The filamentous fungi Trichoderma species produce extracellular cellulase. The current study was carried out to obtain an industrial strain with hyperproduction of cellulase. The wild-type strain, Trichoderma viride TL-124, was subjected to successive mutagenic treatments with UV irradiation, low-energy ion beam implantation, atmospheric pressure non-equilibrium discharge plasma (APNEDP), and N-methyl-N'-nitro-N-nitrosoguanidine to generate about 3000 mutants. Among these mutants, T. viride N879 strain exhibited the greatest relevant activity: 2.38-fold filter paper activity and 2.61-fold carboxymethyl cellulase, 2.18-fold beta-glucosidase, and 2.27-fold cellobiohydrolase activities, compared with the respective wild-type activities, under solid-state fermentation using the inexpensive raw material wheat straw as a substrate. This work represents the first application of APNEDP in eukaryotic microorganisms.